西亚试剂：Proton-Sponge Coated Quantum Dots for siRNA Delivery and In

发布时间：2025-05-19

Proton-Sponge Coated Quantum Dots for siRNA Delivery and Intracellular Imaging

Maksym V. Yezhelyev,^† Lifeng Qi,^‡ Ruth M. O’Regan,^† Shuming Nie,^*†§ and Xiaohu Gao^*‡

Winship Cancer Institute, Emory University, Atlanta, Georgia 30322, Department of Bioengineering, University of Washington, William H. Foege Building N530M, Seattle, Washington 98195, and Department of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 101 Woodruff Circle, Suite 2001, Atlanta, Georgia 30322

Abstract:

We report the rational design of multifunctional nanoparticles for short-interfering RNA (siRNA) delivery and imaging based on the use of semiconductor quantum dots (QDs) and proton-absorbing polymeric coatings (proton sponges). With a balanced composition of tertiary amine and carboxylic acid groups, these nanoparticles are specifically designed to address longstanding barriers in siRNA delivery such as cellular penetration, endosomal release, carrier unpacking, and intracellular transport. The results demonstrate dramatic improvement in gene silencing efficiency by 10−20-fold and simultaneous reduction in cellular toxicity by 5−6-fold, when compared directly with existing transfection agents for MDA-MB-231 cells. The QD−siRNA nanoparticles are also dual-modality optical and electron-microscopy probes, allowing real-time tracking and ultrastructural localization of QDs during delivery and transfection. These new insights and capabilities represent a major step toward nanoparticle engineering for imaging and therapeutic applications.

 

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购

上一篇：西亚试剂:FLA8/KIF3B Phosphorylation Regulates Kinesin-II Interaction
下一篇：西亚试剂：A cross-chiral RNA polymerase ribozyme