西亚试剂：DNA wrapping is required for DNA damage recognition in the

发布时间：2025-11-20

DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway

Hailin Wanga,b, Meiling Lua, Moon-shong Tangc, Bennett Van Houtend,1, J. B. Alexander Rosse, Michael Weinfeldf,2 and X. Chris Lea,2

aDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada T6G 2G3;
bState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
cDepartment of Environmental Medicine, New York University, Tuxedo, NY 10987;
dNational Institute of Environmental Health Sciences, Research Triangle Park, NC 27709;
eDepartment of Chemistry and Biochemistry and BioSpectroscopy Core Research Laboratory, University of Montana, Missoula, MT 59812; and
fExperimental Oncology, Cross Cancer Institute, Edmonton, AB, Canada T6G 1Z2

Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping–melting model supported by results from dynamic monitoring of the key DNA–protein and protein–protein interactions involved in the early stages of the Escherichia coli NER process. Using an analytical technique involving capillary electrophoresis coupled with laser-induced fluorescence polarization, which combines a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli NER. DNA wrapping of UvrB was confirmed by F?rster resonance energy transfer and fluorescence lifetime measurements. This wrapping did not occur with readily denaturable damaged DNA substrates (“bubble” DNA), suggesting that DNA wrapping of UvrB plays an important role in the induction of DNA melting around the damage site. Analysis of DNA wrapping of mutant UvrB Y96A further suggests that a cooperative interaction between DNA wrapping of UvrA2B and contact of the β-hairpin of UvrB with the bulky damage moiety may be involved in the local DNA melting at the damage site.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购

上一篇：重化工密集布局滨海地区加剧了海洋资源环境压力和安全隐患
下一篇：西亚试剂 ：水杨酸-4-叔丁基苯酯