西亚试剂：Characterization of the polyoxin biosynthetic gene cluster

发布时间：2025-11-24

Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H

Wenqing Chen, Tingting Huang, Xinyi He, Qingqing Meng, Delin You, Linquan Bai, Jialiang Li, Mingxuan Wu, Rui li, Zhoujie Xie, Huchen Zhou, Xiufen Zhou, Huarong Tan, and Zixin Deng

Bio-X Life Science Research Center, Shanghai Jiaotong University, Shangahi

A gene cluster (pol) essential for the biosynthesis of polyoxin, a nucleoside antibiotic widely used for the control of phytopathogenic fungi, was cloned from Streptomyces cacaoi. A 46,066-bp region was sequenced and 20 out of 39 of the putative ORFs were defined as necessary for polyoxin biosynthesis as evidenced by its production in a heterologous host, Streptomyces lividans TK24. The role of PolO and PolA in polyoxin synthesis was demonstrated by in vivo experiments, and their functions were unambiguously characterized as O-carbamoyltransferase, and UMP- enolpyruvyl transferase, respectively by in vitro experiments, which enabled making of a modified compound differing slightly from that of early proposed. These studies should provide a solid foundation for the elucidation of the molecular mechanisms for polyoxin biosynthesis, and set the stage for combinatorial biosynthesis using genes encoding different pathways for nucleoside antibiotics.

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