西亚试剂：The Ectopic Expression of Pax4 in the Mouse Pancreas Conver

发布时间：2025-12-04

The Ectopic Expression of Pax4 in the Mouse Pancreas Converts Progenitor Cells into  and Subsequently  Cells

Patrick Collombat1,2,3,11,,,Xiaobo Xu4,Philippe Ravassard3,5,Beatriz Sosa-Pineda6,Sébastien Dussaud5,7,Nils Billestrup8,Ole D. Madsen2,3,9,Palle Serup2,3,9,Harry Heimberg2,3,4andAhmed Mansouri1,2,10,,

1 Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 G?ttingen, Germany
2 Beta Cell Biology Consortium, 2213 Garland Avenue, 9465 MRB IV, Nashville, TN 37323-0494, USA
3 JDRF Center for Beta Cell Therapy in Diabetes, Laarbeeklaan 103, B-1090 Brussels, Belgium
4 Diabetes Research Center, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
5 Biotechnology and Biotherapy Laboratory, Centre de Recherche de l'Institut du Cerveau et de la Moelle, CNRS UMR 7225, INSERM UMRS 975, University Pierre et Marie Curie, H?pital Pitié Salpêtrière, FR-75013 Paris, France
6 Department of Genetics/Tumor Cell Biology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105-3678, USA
7 Centre d'Expérimentation Fonctionnelle, Pitié Salpêtrière Medical Faculty, Université Pierre et Marie Curie, FR-75013 Paris, France
8 Department of Translational Diabetology Research Institute, Hagedorn Niels Steensensvej 6, DK-2820 Gentofte, Denmark
9 Department of Developmental Biology Research Institute, Hagedorn Niels Steensensvej 6, DK-2820 Gentofte, Denmark
10 Department of Clinical Neurophysiology, University of Göttingen, Robert-Koch Strasse 40, D-37075 G?ttingen, Germany

We have previously reported that the loss of Arx and/or Pax4 gene activity leads to a shift in the fate of the different endocrine cell subtypes in the mouse pancreas, without affecting the total endocrine cell numbers. Here, we conditionally and ectopically express Pax4 using different cell-specific promoters and demonstrate that Pax4 forces endocrine precursor cells, as well as mature α cells, to adopt a β cell destiny. This results in a glucagon deficiency that provokes a compensatory and continuous glucagon+ cell neogenesis requiring the re-expression of the proendocrine gene Ngn3. However, the newly formed β cells fail to correct the hypoglucagonemia since they subsequently acquire a β cell phenotype upon Pax4 ectopic expression. Notably, this cycle of neogenesis and redifferentiation caused by ectopic expression of Pax4 in β cells is capable of restoring a functional β cell mass and curing diabetes in animals that have been chemically depleted of β cells.

 

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购

 

上一篇：11家药企进入浙江百强
下一篇：西亚试剂：蛋白质组水平上绘制二硫键图谱