西亚试剂：Deficiency in RNA editing enzyme ADAR2 impairs regulated ex

发布时间：2026-01-26

Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis
Liu Yang*,1, Liyun Zhao*,1, Zhenji Gan*,2, Zixuan He,3, Jingyue Xu, Xiang Gao, Xiaorui Wang, Weiping Han, Liangyi Chen, Tao Xu, Wenjun Li*,4 and Yong Liu*,4

* Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences (SIBS), Shanghai, China;
 Institute of Biophysics, Chinese Academy of Sciences (CAS), Beijing, China;
 Model Animal Research Center, Nanjing University, Nanjing, China; and
 Singapore Bioimaging Consortium, Agency for Science, Technology, and Research (A*STAR), Singapore

Mammalian RNA editing catalyzed by adenosine deaminases acting on RNA (ADARs) ADAR1 and ADAR2 plays pivotal roles in the brain through functional modifications of neurotransmitter receptors and ion channels. We have demonstrated previously that RNA editing by ADAR2 is regulated metabolically in pancreatic β cells. To investigate the cellular functions of ADAR2 in professional secretory cells, we studied the effects of ADAR2 knockdown on regulated exocytosis. Selective knockdown of ADAR2 expression markedly impaired glucose-stimulated insulin secretion in the rat insulinoma INS-1 cells and primary pancreatic islets and significantly diminished KCl-stimulated secretion of exogenous human growth hormone or endogenous chromogranin B protein in the rat adrenal pheochromocytoma PC12 cells. Notably, restored overexpression of catalytically active but not editing-deficient mutant ADAR2 could rescue the impairment in stimulated secretion from ADAR2 knockdown cells. Moreover, ADAR2 suppression significantly attenuated Ca2+-evoked membrane capacitance increases and appreciably reduced the number of membrane-docked insulin granules in INS-1 cells. Interestingly, the secretory defects resulting from ADAR2 deficiency were coupled to decreased expression of Munc18-1 and synaptotagmin-7, two key molecules in the regulation of vesicle exocytosis. Thus, these findings reveal an important aspect of ADAR2 actions in regulated exocytosis, implicating RNA editing in the control of cellular secretory machinery.—Yang, L., Zhao, L., Gan, Z., He, Z., Xu, J., Gao, X., Wang, X., Han, W., Chen, L., Xu, T., Li, W., Liu, Y. Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis.

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