西亚试剂：Crystal structures of the Lsm complex bound to the 3′ end s

发布时间：2025-06-03

Crystal structures of the Lsm complex bound to the 3′ end sequence of U6 small nuclear RNA

Lijun Zhou，Jing Hang，Yulin Zhou，Ruixue Wan，Guifeng Lu，Ping Yin，Chuangye Yan& Yigong Shi

Splicing of precursor messenger RNA (pre-mRNA) in eukaryotic cells is carried out by the spliceosome1， which consists of five small nuclear ribonucleoproteins (snRNPs) and a number of accessory factors and enzymes2. Each snRNP contains a ring-shaped subcomplex of seven proteins and a specific RNA molecule2， 3， 4. The U6 snRNP contains a unique heptameric Lsm protein complex， which specifically recognizes the U6 small nuclear RNA at its 3′ end. Here we report the crystal structures of the heptameric Lsm complex， both by itself and in complex with a 3′ fragment of U6 snRNA， at 2.8？？ resolution. Each of the seven Lsm proteins interacts with two neighbouring Lsm components to form a doughnut-shaped assembly， with the order Lsm3–2–8–4–7–5–6. The four uridine nucleotides at the 3′ end of U6 snRNA are modularly recognized by Lsm3， Lsm2， Lsm8 and Lsm4， with the uracil base specificity conferred by a highly conserved asparagine residue. The uracil base at the extreme 3′ end is sandwiched by His？36 and Arg？69 from Lsm3， through π–π and cation–π interactions， respectively. The distinctive end-recognition of U6 snRNA by the Lsm complex contrasts with RNA binding by the Sm complex in the other snRNPs. The structural features and associated biochemical analyses deepen mechanistic understanding of the U6 snRNP function in pre-mRNA splicing.

 

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购

上一篇：仪器仪表是生产生活的基础行业
下一篇：西亚试剂:Gilead Sciences Harvoni Has A Better-Than-Expected Launch