西亚试剂：Phospho-Regulated Interaction between Kinesin-6 Klp9p and M

发布时间：2025-06-12

Phospho-Regulated Interaction between Kinesin-6 Klp9p and Microtubule Bundler Ase1p Promotes Spindle Elongation

Chuanhai Fu ¹,Jonathan J. Ward ²,Isabelle Loiodice ^1,3,Guilhem Velve-Casquillas ^1,3, Francois J. Nedelec ^2,*, and Phong T. Tran ^1,3,*

1 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 10104, USA
2 Cell Biology and Biophysics Program, EMBL, Heidelberg 69117, Germany
3 Institut Curie, CNRS UMR144, Paris 75005, France
* Corresponding author

Summary

The spindle midzone composed of antiparallel microtubules, microtubule-associated proteins (MAPs), and motors is the structure responsible for microtubule organization and sliding during anaphase B. In general, MAPs and motors stabilize the midzone and motors produce sliding. We show that fission yeast kinesin-6 motor klp9p binds to the microtubule antiparallel bundler ase1p at the midzone at anaphase B onset. This interaction depends upon the phosphorylation states of klp9p and ase1p. The cyclin-dependent kinase cdc2p phosphorylates and its antagonist phosphatase clp1p dephosphorylates klp9p and ase1p to control the position and timing of klp9p-ase1p interaction. Failure of klp9p-ase1p binding leads to decreased spindle elongation velocity. The ase1p-mediated recruitment of klp9p to the midzone accelerates pole separation, as suggested by computer simulation. Our findings indicate that a phosphorylation switch controls the spatial-temporal interactions of motors and MAPs for proper anaphase B, and suggest a mechanism whereby a specific motor-MAP conformation enables efficient microtubule sliding.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购

 

上一篇：西亚试剂：专产技术将弥补全球丙烯缺口
下一篇：西亚试剂：RAB-Like 2 Has an Essential Role in Male Fertility, Sperm I