西亚试剂：：Oxidation-sensing Regulator AbfR Regulates Oxidative

发布时间：2025-06-19

Xing Liu1, Xiaoxu Sun1, Youcong Wu2, Cen Xie1, Wenru Zhang1, Dan Wang3, Xiaoyan Chen1, Di Qu2, Jianhua Gan2, Hao Chen3, Hualiang Jiang1, Lefu Lan1 and Cai-Guang Yang1,*

Staphylococcus epidermidis is a notorious human pathogen that is the major cause of infections related to implanted medical devices. Although redox regulation involving reactive oxygen species (ROS) is now recognized as a critical component of bacterial signaling and regulation, the mechanism by which S. epidermidis senses and responds to oxidative stress remains largely unknown. Here, we report a new oxidation-sensing regulator, AbfR (Aggregation and Biofilm Formation Regulator) in S. epidermidis. An environment of oxidative stress mediated by hydrogen peroxide (H2O2) or cumene hydroperoxide (CHP) markedly up-regulates the expression of abfR gene. Similar to Pseudomonas aeruginosa OspR, AbfR is negatively auto-regulated and dissociates from promoter DNA in the presence of oxidants. In vivo and in vitro analyses indicate that Cys-13 and Cys-116 are the key functional residues to form an intersubunit disulphide bond upon oxidation in AbfR. We further show that deletion of abfR leads to a significant induction in H2O2 or CHP resistance, enhanced bacterial aggregation, and reduced biofilm formation. These effects are mediated by de-repression of SERP2195 and gpxA-2 that lie immediately downstream of the abfR gene in the same operon. Thus, oxidative stress likely acts as a signal to modulate S. epidermidis key virulence properties through AbfR.

丙烯醛肉桂醛桂皮醛4-硝基肉桂醛

上一篇：邻苯二甲酸氢钾生产方法
下一篇：α-蒎烯生产方法