西亚试剂:Splicing Factors Facilitate RNAi-Directed Silencing in Fiss
发布时间:2025-06-22
Splicing Factors Facilitate RNAi-Directed Silencing in Fission Yeast
Elizabeth H. Bayne,1 Manuela Portoso,1* Alexander Kagansky,1 Isabelle C. Kos-Braun,1 Takeshi Urano,2 Karl Ekwall,3 Flavia Alves,1 Juri Rappsilber,1 Robin C. Allshire1
Heterochromatin formation at fission yeast centromeres is directed by RNA interference (RNAi). Noncoding transcripts derived from centromeric repeats are processed into small interfering RNAs (siRNAs) that direct the RNA-induced transcriptional silencing (RITS) effector complex to engage centromere transcripts, resulting in recruitment of the histone H3 lysine 9 methyltransferase Clr4, and hence silencing. We have found that defects in specific splicing factors, but not splicing itself, affect the generation of centromeric siRNAs and consequently centromeric heterochromatin integrity. Moreover, splicing factors physically associate with Cid12, a component of the RNAi machinery, and with centromeric chromatin, consistent with a direct role in RNAi. We propose that spliceosomal complexes provide a platform for siRNA generation and hence facilitate effective centromere repeat silencing.
1 Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, 6.34 Swann Building, Edinburgh EH9 3JR, UK.
2 Department of Biochemistry, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo 693-8501, Japan.
3 Karolinska Institute, Department of Biosciences and Medical Nutrition, University College Sodertorn, Novum 141, 57 Huddinge, Sweden.
- 以上资料由西亚试剂:http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询
- 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购
下一篇:西亚试剂:2-氟吡啶