西亚试剂：Genomic DNA Quickprep for PCR

发布时间：2025-07-11

1. macerate tissue in Eppendorf tube without butter at RT
2. add 400 ml extraction buffer
3. vortex for 4 sec
4. leave sample at RT until other samples are ready (> 1 h)
5. spin in microfuge for 1 min
6. transfer 300 ml of supernatant to different Eppendorf tube (prefilled with 300 ml isopropanole)
7. mix and leave at RT for 2 min
8. spin for 5 min
9. vacuum dry pellet and take up in 100 ml TE
10. use 1-2.5 ml for PCR

 

Remarks:

DNA is stable for one year at 4°C

 

Solutions:

Extraction buffer: 200 mM Tris-HCl pH 7.5 250 mM NaCl  25 mM EDTA 0.5%  SDS

 

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