西亚试剂：：Copy Number Variation of Multiple Genes at Rhg1

发布时间：2025-07-26

David E. Cook1,*, Tong Geon Lee2,*, Xiaoli Guo1,*,†, Sara Melito1,‡, Kai Wang3, Adam Bayless1, Jianping Wang2,§, Teresa J. Hughes1, David K. Willis4, Thomas Clemente5, Brian W. Diers2, Jiming Jiang3, Matthew E. Hudson2,6,¶, Andrew F. Bent1,¶#

 

The rhg1-b allele of soybean is widely used for resistance against soybean cyst nematode (SCN), the most economically damaging pathogen of soybeans in the United States. Gene silencing showed that genes in a 31-kb segment at rhg1-b, encoding an amino acid transporter, an α-SNAP protein, and a WI12 (wound-inducible domain) protein, each contribute to resistance. There is one copy of the 31-kb segment per haploid genome in susceptible varieties, but 10 tandem copies in the rhg1-b haplotype. Overexpression of the individual genes in roots was ineffective, but overexpression of the genes together conferred enhanced SCN resistance. Hence Rhg1-mediated SCN resistance is conferred by copy number variation that increases the expression of a set of dissimilar genes in a repeated multi-gene segment.

丙烯醛肉桂醛桂皮醛4-硝基肉桂醛

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