Template-Directed Synthesis in 3′- and 5′-Direction with Reversible Termination

Dipl.-Chem. Andreas Kaiser1, Dipl.-Chem. Sebastian Spies1, Dr. Tanja Lommel2, Prof. Clemens Richert

A method for DNA-templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′-extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′-extension).

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