西亚试剂：MicroRNA Profiling Reveals Two Distinct p53-Related Human P

发布时间：2025-07-30

MicroRNA Profiling Reveals Two Distinct p53-Related Human Pluripotent Stem Cell States

Authors

Pierre Neveu, Min Jeong Kye, Shuping Qi, David E. Buchholz, Dennis O. Clegg, Mustafa Sahin, In-Hyun Park, Kwang-Soo Kim, George Q. Daley, Harley I. Kornblum, Boris I. Shraiman, Kenneth S. Kosik

Highlights

miRNA profiles distinguish two categories of human pluripotent stem cells

The p53 network status distinguishes pluripotent cells independently of their origin

p53-targeting miRNAs change the classification status of iPSCs

A 2D representation of miRNA profiles tracks differentiation and reprogramming

Summary

Reprogramming methodologies have provided multiple routes for achieving pluripotency. However, pluripotency is generally considered to be an almost singular state, with subtle differences described between induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). We profiled miRNA expression levels across 49 human cell lines, including ESCs, iPSCs, differentiated cells, and cancer cell lines. We found that the resulting miRNA profiles divided the iPSCs and hESCs examined into two distinct categories irrespective of the cell line origin. The miRNAs that defined these two pluripotency categories also distinguished cancer cells from differentiated cells. Transcriptome analysis suggested that several gene sets related to p53 distinguished these categories, and overexpression of the p53-targeting miRNAs miR-92 and miR-141 in iPSCs was sufficient to change their classification status. Thus, our results suggest a subdivision of pluripotent stem cell states that is independent of their origin but related to p53 network status.

 

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