西亚试剂：Role of ERO1-α–mediated stimulation of inositol 1,4,5-triph

发布时间：2025-08-18

Role of ERO1-α–mediated stimulation of inositol 1,4,5-triphosphate receptor activity in endoplasmic reticulum stress–induced apoptosis

Gang Li1, Marco Mongillo2,4, King-Tung Chin5, Heather Harding5, David Ron5, Andrew R. Marks2,4, and Ira Tabas1,2,3,4

1 Department of Medicine, 2 Department of Physiology and Cellular Biophysics, 3 Department of Pathology and Cell Biology, and 4 Clyde and Helen Wu Center for Molecular Cardiology, Columbia University, New York, NY 10032 5 Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016

Endoplasmic reticulum (ER) stress–induced apoptosis is involved in many diseases, but the mechanisms linking ER stress to apoptosis are incompletely understood. Based on roles for C/EPB homologous protein (CHOP) and ER calcium release in apoptosis, we hypothesized that apoptosis involves the activation of inositol 1,4,5-triphosphate (IP3) receptor (IP3R) via CHOP-induced ERO1- (ER oxidase 1 ). In ER-stressed cells, ERO1- is induced by CHOP, and small interfering RNA (siRNA) knockdown of ERO1- suppresses apoptosis. IP3-induced calcium release (IICR) is increased during ER stress, and this response is blocked by siRNA-mediated silencing of ERO1- or IP3R1 and by loss-of-function mutations in Ero1a or Chop. Reconstitution of ERO1- in Chop–/– macrophages restores ER stress–induced IICR and apoptosis. In vivo, macrophages from wild-type mice but not Chop–/– mice have elevated IICR when the animals are challenged with the ER stressor tunicamycin. Macrophages from insulin-resistant ob/ob mice, another model of ER stress, also have elevated IICR. These data shed new light on how the CHOP pathway of apoptosis triggers calcium-dependent apoptosis through an ERO1-–IP3R pathway.

 

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购

 

 

上一篇：西亚试剂:23andMe的基因诊断到底靠不靠谱
下一篇：吲哚丁酸制法/工艺