西亚试剂：SSB protein diffusion on single-stranded DNA stimulates Rec

发布时间：2025-09-15

SSB protein diffusion on single-stranded DNA stimulates RecA filament formation

Rahul Roy1,2,5, Alexander G. Kozlov3, Timothy M. Lohman3 & Taekjip Ha1,2,4

1 Center for Biophysics and Computational Biology,
2 Department of Physics and Center for the Physics of Living Cells, University of Illinois, Urbana-Champaign, Illinois 61801, USA
3 Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
4 Howard Hughes Medical Institute, Urbana, Illinois 61801, USA
5 Present address: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Correspondence to: Taekjip Ha1,2,4 Correspondence and requests for materials should be addressed to T.H.

Single-stranded DNA generated in the cell during DNA metabolism is stabilized and protected by binding of ssDNA-binding (SSB) proteins. Escherichia coli SSB, a representative homotetrameric SSB, binds to ssDNA by wrapping the DNA using its four subunits. However, such a tightly wrapped, high-affinity protein–DNA complex still needs to be removed or repositioned quickly for unhindered action of other proteins. Here we show, using single-molecule two- and three-colour fluorescence resonance energy transfer, that tetrameric SSB can spontaneously migrate along ssDNA. Diffusional migration of SSB helps in the local displacement of SSB by an elongating RecA filament. SSB diffusion also melts short DNA hairpins transiently and stimulates RecA filament elongation on DNA with secondary structure. This observation of diffusional movement of a protein on ssDNA introduces a new model for how an SSB protein can be redistributed, while remaining tightly bound to ssDNA during recombination and repair processes.

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