西亚试剂：Refoldability and Thermoactivity of Fluorinated Phosphotrie

发布时间：2025-05-13

ChemBioChem   DOI： 10.1002/cbic.201100221

Enhanced Refoldability and Thermoactivity of Fluorinated Phosphotriesterase

Baker, Peter James; Montclare, Jin Kim

Keywords:biotechnology;enzyme catalysis;noncanonical amino acids;phosphotriesterase;protein engineeringTeflon‐like globular proteins: Global incorporation of p‐fluorophenylalanine (pFF) in the S phosphotriesterase dimer (see figure) was found to stabilize the protein and led to refoldability and improved activity for substrates at elevated temperatures.

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