西亚试剂：Conserved C-Terminal Motifs Required for Avirulence and Sup

发布时间：2025-10-07

Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by Phytophthora sojae effector Avr1b

Daolong Dou ¹, Shiv D. Kale ¹, Xinle Wang ², Yubo Chen ², Qunqing Wang ², Xia Wang¹, Rays H.Y. Jiang ³, Felipe D. Arredondo ¹, Ryan G. Anderson ⁴, Poulami B. Thakur⁴, John M. McDowell ⁴, Yuanchao Wang ², and Brett M. Tyler

¹ Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061
² Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
³ Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061; Laboratory of Phytopathology, Wageningen University, NL-6709 PD Wageningen, The Netherlands
⁴ Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061
⁵ Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061; Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

 

The sequenced genomes of oomycete plant pathogens contain large superfamilies of effector proteins containing the protein translocation motif RXLR-dEER. However, the contributions of these effectors to pathogenicity remain poorly understood. Here, we show that the Phytophthora sojae effector protein Avr1b can contributepositively to virulence and can suppress programmed cell death (PCD) triggered by the mouse BAX protein in yeast, soybean (Glycine max), and Nicotiana benthamianacells. We identify three conserved motifs (K, W, and Y) in the C terminus of the Avr1b protein and show that mutations in the conserved residues of the W and Y motifs reduce or abolish the ability of Avr1b to suppress PCD and also abolish the avirulence interaction of Avr1b with the Rps1b resistance gene in soybean. W and Y motifs are present in at least half of the identified oomycete RXLR-dEER effectorcandidates, and we show that three of these candidates also suppress PCD in soybean. Together, these results indicate that the W and Y motifs are critical for the interaction of Avr1b with host plant target proteins and support the hypothesis that these motifs are critical for the functions of the very large number of predicted oomycete effectors that contain them.

 

 

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