西亚试剂：Ago2 facilitates Rad51 recruitment and DNA double-strand br

发布时间：2025-10-08

Ago2 facilitates Rad51 recruitment and DNA double-strand break repair by homologous recombination

Min Gao1，4， Wei Wei2，3， Ming-Ming Li1，4， Yong-Sheng Wu1， Zhaoqing Ba2，3， Kang-Xuan Jin1，4， Miao-Miao Li1，4， You-Qi Liao1，4， Samir Adhikari1，4， Zechen Chong1， Ting Zhang1， Cai-Xia Guo1， Tie-shan Tang5， Bing-Tao Zhu6， Xing-Zhi Xu6， Niels Mailand7， Yun-Gui Yang1，4， Yijun Qi2，3 and Jannie M Rendtlew Danielsen1，7

DNA double-strand breaks (DSBs) are highly cytotoxic lesions and pose a major threat to genome stability if not properly repaired. We and others have previously shown that a class of DSB-induced small RNAs (diRNAs) is produced from sequences around DSB sites. DiRNAs are associated with Argonaute (Ago) proteins and play an important role in DSB repair， though the mechanism through which they act remains unclear. Here， we report that the role of diRNAs in DSB repair is restricted to repair by homologous recombination (HR) and that it specifically relies on the effector protein Ago2 in mammalian cells. Interestingly， we show that Ago2 forms a complex with Rad51 and that the interaction is enhanced in cells treated with ionizing radiation. We demonstrate that Rad51 accumulation at DSB sites and HR repair depend on catalytic activity and small RNA-binding capability of Ago2. In contrast， DSB resection as well as RPA and Mre11 loading is unaffected by Ago2 or Dicer depletion， suggesting that Ago2 very likely functions directly in mediating Rad51 accumulation at DSBs. Taken together， our findings suggest that guided by diRNAs， Ago2 can promote Rad51 recruitment and/or retention at DSBs to facilitate repair by HR.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购

上一篇：清华三农论坛2017在清华大学举行
下一篇：西亚试剂：菲