西亚试剂：Histone Crosstalk between H2B Monoubiquitination and H3 Met

发布时间：2025-10-12

Histone Crosstalk between H2B Monoubiquitination and H3 Methylation Mediated by COMPASS

Jung-Shin Lee,1 Abhijit Shukla,2 Jessica Schneider,1 Selene K. Swanson,1 Michael P. Washburn,1 Laurence Florens,1 Sukesh R. Bhaumik,2 and Ali Shilatifard1,

1 Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
2 Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Drive, Carbondale, IL 62901, USA

Corresponding author
Ali Shilatifard
COMPASS, the yeast homolog of the mammalian MLL complex, is a histone H3 lysine 4 (H3K4) methylase consisting of Set1 (KMT2) and seven other polypeptides, including Cps35, the only essential subunit. Histone H2B monoubiquitination by Rad6/Bre1 is required for both H3K4 methylation by COMPASS, and H3K79 methylation by Dot1. However, the molecular mechanism for such histone crosstalk is poorly understood. Here, we demonstrate that histone H2B monoubiquitination controls the binding of Cps35 with COMPASS complex. Cps 35 is required for COMPASS' catalytic activity in vivo, and the addition of exogenous purified Cps35 to COMPASS purified from a Δrad6 background results in the generation of a methylation competent COMPASS. Cps35 associates with the chromatin of COMPASS-regulated genes in a H2BK123 monoubiquitination-dependent but Set1-independent manner. Cps35 is also required for proper H3K79 trimethylation. These findings offer insight into the molecular role of Cps35 in translating the H2B monoubiquitination signal into H3 methylation.

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