西亚试剂：High-throughput microfluidic single-cell RT-qPCR

发布时间：2025-10-28

High-throughput microfluidic single-cell RT-qPCR

White, Adam K.; VanInsberghe, Michael; Petriv, Oleh I.; Hamidi, Mani; Sikorski, Darek; Marra, Marco A.; Piret, James; Aparicio, Samuel; Hansen, Carl L.

A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysisof transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precisionRT-qPCR measurements of gene expression from hundreds of single cells per run. Our device executes all steps of single-cellprocessing, including cell capture, cell lysis, reverse transcription, and quantitative PCR. In addition to higher throughputand reduced cost, we show that nanoliter volume processing reduced measurement noise, increased sensitivity, and providedsingle nucleotide specificity. We apply this technology to 3,300 single-cell measurements of (i) miRNA expression in K562 cells, (ii) coregulation of a miRNA and one of its target transcripts during differentiation in embryonic stem cells, and (iii) single nucleotide variant detection in primary lobular breast cancer cells. The core functionality established here providesthe foundation from which a variety of on-chip single-cell transcription analyses will be developed.

 

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购

上一篇：通过项目推动产业升级，通过项目推动跨越式发展
下一篇：西亚试剂：Molecular Cell：Mrc基因对DNA复制的作用